Construction and identification of the recombinant plasmid pET30a-EgA31-Eg95 of Echinococcus granulosus
نویسندگان
چکیده
منابع مشابه
Construction and identification of the recombinant plasmid pET30a-EgA31-Eg95 of Echinococcus granulosus
To clone the Eg95 and EgA31 antigen genes into the prokaryotic expression plasmid pET30a-EgA31-Eg95, we expressed the recombinant protein EgA31-Eg95 and confirmed with western blot analysis. The total RNA was extracted from the protoscoleces of Echinococcus granulosus (E. granulosus) adult worms. The complementary DNA (cDNA) encoding the EgA31 antigen was amplified via quantitative real-time po...
متن کاملConstruction and Identification of a Recombinant Plasmid Encoding Echinococcus granulosus Oncosphere Antigen (EG95)
Background Cystic echinococcosis (CE), as a zoonotic disease cause to health threat and economic losses. Despite implemented control programs, few countries have been able to decrease or eliminate this infection. Vaccination of the intermediate host offers an additional strategy to control the parasite transmission and EG95 antigen is considered more than the others in the vaccine issue. Accord...
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Molecular Cloning and Expression of EG95 Gene of Iranian Isolates of Echinococcus granulosus
BACKGROUND Echinococcosis or hydatidosis is a chronic, zoonotic worldwide infection that occurs by the larval stages of taeniid cestodes of the genus Echinococcus. Iran is known as endemic region for this infection in the world. Vaccination has been considered as a good prevention method for this disease. Recombinant vaccines containing EG95 protein, against E. granulosus, has shown a high degr...
متن کاملEvaluation of Immunogenicity of Novel Isoform of EG95 (EG95-5G1) From Echinococcus granulosus in BALB/C Mice
BACKGROUND Echinococcosis is a zoonotic parasitic disease of humans and various herbivorous domestic animals transmitted by the contact with domestic and wild carnivores, mainly dogs and foxes. The aim of this study is the production, purification and evaluation immunogenicity of new construction of EG95 protein. METHODS The recombinant plasmid pET32-a+ used for Eg95 expression was constructe...
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ژورنال
عنوان ژورنال: Experimental and Therapeutic Medicine
سال: 2013
ISSN: 1792-0981,1792-1015
DOI: 10.3892/etm.2013.1393